Chemical decarboxylation kinetics and identification of amino acid standards by benchtop NMR spectroscopy

Abstract In this paper, we examined the competence of amino acids as standards for instrumental biochemical analysis. The chosen were first dissolved in various aquatic solutions and then measured a benchtop NMR spectrometer, which is not common choice such analytical investigations. Analysis by mass spectrometry was used addition. As part these investigations, determined stability ornithine, glutamic acid, alanine, glycine, proline, pyroglutamic phenylalanine trans-4-hydroxy-D-proline under critical basic acidic pH conditions other conditions. We observed that all acid remain stable given chemical transformation takes place. Given our findings spectroscopy, additional kinetic measurements carried out with spectrometer. discovered becomes unstable decarboxylates to pyrrolidone.